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insertional mutagenesis
Insertional mutagenesis is mutagenesis of DNA by the insertion of one or more bases.〔(Biology-online )〕
Insertional mutations can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab.
== Signature tagged mutagenesis ==
(詳細はgenes. A transposon, such as the ''Drosophila melanogaster'' P element, is allowed to integrate at random locations in the genome of the organism being studied. Mutants generated by this method are then screened for any unusual phenotypes. If such a phenotype is found then it can be assumed that the insertion has caused the gene relating to the usual phenotype to be inactivated. Because the sequence of the transposon is known, the gene can be identified, either by sequencing the whole genome and searching for the sequence, or using the polymerase chain reaction to amplify specifically that gene.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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